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KMID : 0359320170570040223
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Korean Journal of Veterinary Research
2017 Volume.57 No. 4 p.223 ~ p.226
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Development of high-performance liquid chromatography methods for the anticoccidials: toltrazuril and diclazuril
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Jung Kyung-Hun
Jeong Mi-Young
Park Hae-Chul
Hossain Md Akil
Kim Dae-Gyun
Lee Kwang-Jick
Kang Jeong-Woo
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Abstract
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This study was undertaken to develop new analytical methods for assessment of anticoccidials. Highperformance liquid chromatography (HPLC) was found to be a fast, reliable, and practical method. The anticoccidials used in this experiment were toltrazuril and diclazuril, and the analysis factors were specificity, linearity, accuracy, repeatability, and intermediate precision. The linearity of each anticoccidial was better than 0.99, and the accuracies were 99.5% and 99.1% with relative SD of 0.5 and 0.4, respectively. To assess whether the developed HPLC method could be effectively applied, toltrazuril and diclazuril post-market veterinary products (five products) that are currently sold were tested. The results revealed no non-compliant items and the method was applied successfully. Therefore, the newly developed HPLC method for anticoccidial assessment described in this study may be useful as a reference method in the Korean Standards of Veterinary Pharmaceuticals for the analysis of toltrazuril and diclazuril.
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KEYWORD
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coccidiostats, diclazuril, high performance liquid chromatography, toltrazuril
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